Ana Paula Zacarias is a Portuguese diplomat and the former Secretary of State for European Affairs of Portugal. She has a degree in Cultural Anthropology from the Universidade Nova de Lisboa and has served in various positions in the Portuguese Ministry of Foreign Affairs and the European Union.

Ms. Zacarias was the Ambassador of Portugal to Estonia from 2005 to 2008, the Head of the European Union's delegation to Brazil from 2011 to 2015, and the EU Ambassador to Ecuador and Colombia from 2015 to 2017. She was also a member of the Executive Committee of the Conference on the Future of Europe, which started in 2021.

Since 2022, she has been the Permanent Representative of Portugal to the United Nations, where she presented her credentials to UN Secretary-General António Guterres. She is a recipient of several honors and awards, including the Order of Merit and the Order of Freedom from Portugal.